Kvalifikation: Lørdag den 5. juni 2010 - Kl. 13.00 - 16.00 på TV3 Puls
VM-afdeling: Søndag den 6. juni 2010 - Kl. 10.45 - 15.20 på TV3 Puls
Sted: Mugello, Italy
Tidszone: GMT+1
Koordinater: 43°59′51″N 11°22′19″E / 43.9975°N 11.37194°E / 43.9975; 11.37194
Længde: 5245 m
Sving: 15
Bedste omgangstid: 1:48:130 (Valentino Rossi, Fiat Yamaha, 2008)
Startopstilling Mugello.:
01 26 Dani PEDROSA SPA Repsol Honda Team Honda 329.3 1'48.819
02 99 Jorge LORENZO SPA Fiat Yamaha Team Yamaha 324.8 1'48.996 0.177 / 0.177
03 27 Casey STONER AUS Ducati Marlboro Team Ducati 339.3 1'49.432 0.613 / 0.436
04 69 Nicky HAYDEN USA Ducati Marlboro Team Ducati 336.4 1'49.546 0.727 / 0.114
05 5 Colin EDWARDS USA Monster Yamaha Tech 3 Yamaha 323.9 1'49.683 0.864 / 0.137
06 14 Randy DE PUNIET FRA LCR Honda MotoGP Honda 323.9 1'49.737 0.918 / 0.054
07 11 Ben SPIES USA Monster Yamaha Tech 3 Yamaha 320.4 1'49.861 1.042 / 0.124
08 4 Andrea DOVIZIOSO ITA Repsol Honda Team Honda 327.3 1'50.065 1.246 / 0.204
09 41 Aleix ESPARGARO SPA Pramac Racing Team Ducati 335.2 1'50.168 1.349 / 0.103
10 7 Hiroshi AOYAMA JPN Interwetten Honda MotoGP Honda 330.4 1'50.224 1.405 / 0.056
11 58 Marco SIMONCELLI ITA San Carlo Honda Gresini Honda 327.1 1'50.434 1.615 / 0.210
12 65 Loris CAPIROSSI ITA Rizla Suzuki MotoGP Suzuki 322.8 1'50.479 1.660 / 0.045
13 40 Hector BARBERA SPA Paginas Amarillas Aspar Ducati 341.2 1'50.561 1.742 / 0.082
14 33 Marco MELANDRI ITA San Carlo Honda Gresini Honda 328.9 1'50.664 1.845 / 0.103
15 36 Mika KALLIO FIN Pramac Racing Team Ducati 338.5 1'50.970 2.151 / 0.306
16 19 Alvaro BAUTISTA SPA Rizla Suzuki MotoGP Suzuki 324.7 1'53.243 4.424 / 2.273